The study aimed to investigate whether R, S-1,3-butanediol diacetoacetate (BD-AcAc2), a ketone ester, increases energy expenditure and markers of adipose tissue thermogenesis in mice with HFD-induced obesity. The results showed that BD-AcAc2 increased resting energy expenditure and markers of brown and white adipose thermogenesis in obese mice, suggesting its potential as a therapeutic intervention for obesity.